Image of Daniel A. Blasiole, Ph.D.

Daniel A. Blasiole, Ph.D.

Registered Patent Agent
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  • Ph.D., Biochemistry, University of Wisconsin – Madison 
  • M.A., Philosophy of Science, University of California at San Diego
  • B.A., Philosophy with Pre-Medical Curriculum, Franklin & Marshall College


  • U.S. Patent & Trademark Office (USPTO)

Your patent counsel needs to understand your technology.  As a registered patent agent, Dan brings to his practice a detailed understanding of experimental science, focused expertise in a number of biotechnological disciplines and extensive, hands-on research experience.   Dan works with university technology transfer organizations, individual inventors, and companies ranging from startups to multi-national corporations in securing their patent rights both domestically and internationally and in managing their patent portfolios.

He conducts nearly all aspects of practice before the United States Patent and Trademark Office, including patent prosecution, requests for reexamination, and appeals before the Patent Trial and Appeal Board. In addition, Dan advises on such matters as invalidity opinions, freedom to operate opinions, patentability opinions, licensing agreements, and litigation.

Dan has experience with a number of technologies, including enzymes, antibodies, medical devices, pharmaceutical compounds, cellular reprogramming methods, assays, assay platforms, nutraceutical compositions, disease treatments, nanopositioning systems, chemical manufacturing processes, and mechanical devices, among others.

His practice is informed with his expertise in a number of areas of bioscience, such as biochemistry, cell biology, molecular biology, microbiology, genetics, and genomics. He has researched molecular and cellular mechanisms underlying metabolic disease, particularly the role of certain protein receptors and enzymes in regulating lipid metabolism. He has also contributed to a genetic mapping of mRNA expression and metabolic profiles in an F2 intercross between diabetes-resistant and diabetes-susceptible mouse strains. In addition, he has conducted molecular genetic research on microbial pathogens and developed multiplex real-time PCR viral diagnostic arrays for the U.S. Department of Defense.

Articles & Presentations

  • Wisconsin Attorneys Desk Reference – Patent Chapter (with Charles Sara and Navid Fanaeian)

Awards & Recognitions

  • Journal of Biological Chemistry cover image, 2008, Apr. 25; 283(17)
  • NIH National Research Service Award, UW Institute on Aging
  • NIH National Research Service Award, UW Cardiovascular Research Center
  • Salutatorian (class rank of 2) of undergraduate graduating class
  • Phi Beta Kappa

Client Recommendations

“This is really brilliant…. You have won my business.” – CEO and founder of a biotechnology company, regarding a patent application directed to polymerase enzymes.

“I was amazed at how thorough a job you did. I could not really come up with any substantial additions to the information that we have talked about.” – Surgeon and inventor, regarding a patent application directed to a medical device.

“Kudos to the author of the main body. It is well written, so I have very few comments. Actually, I think we should talk about using it as the basis for a review article.” – University professor and inventor, regarding a patent application directed to genetically recombinant microorganisms.


Dan works with university technology transfer organizations, individual inventors, and companies ranging from startups to multi-national corporations in securing their patent rights both domestically and internationally and in managing their patent portfolios.


  • U.S. Patent 8,343,545
    Method of producing microparticles
    University College London
  • U.S. Patent 8,574,898Translation-coupling systems
    Wisconsin Alumni Research Foundation
  • U.S. Patent 8,569,465
    Method for modifying lignin structure using monolignol ferulate conjugates
    Wisconsin Alumni Research Foundation and U.S. Department of Agriculture
  • U.S. Patent 8,568,448
    Clotting cascade initiating apparatus and methods of use and methods of closing wounds
    CloSys Corporation
  • U.S. Patent 8,501,220
    Stabilized liquid egg material for extended shelf life
    R&D Lifesciences
  • U.S. Patent 8,461,136
    Compositions and methods for protecting cells during cancer chemotherapy and radiotherapy
    Wisconsin Alumni Research Foundation
  • U.S. Patent 8,431,174
    Method to separate lipids from cheese whey
    Wisconsin Alumni Research Foundation
  • U.S. Patent 8,410,326
    Integrated process and apparatus to produce hydrocarbons from aqueous solutions of lactones, hydroxy-carboxylic acids, alkene-carboxylic acids, and/or alcohols
    Wisconsin Alumni Research Foundation
  • U.S. Patent 8,404,890
    Production of 2,4-hexadienoic acid and 1,3-pentadiene from 6-methyl-5,6-dihydro-2-pyrone
    Wisconsin Alumni Research Foundation
  • U.S. Patent 8,258,188
    Method of using punicic acid to enhance immune response and prevent metabolic disorders
    Nutrition Therapeutics, Inc.
  • U.S. Patent 8,252,732
    Hydrophilic surfaces for reducing friction forces
    Bayer Material Science AG
  • U.S. Patent 8,211,906
    Method of inhibiting growth of neoplastic cells and inhibiting infection by administering an immune enhancer drug. 
    Lawrence C. Scherrer, M.D. 
  • U.S. Patent 8,211,487
    Inhibition of ice crystal growth
    Srinivasan Damodaran, Ph.D.
  • U.S. Patent 8,173,717
    Silicone foams, process to make them and applications thereof
    Zotefoams, PLC
  • U.S. Patent 8,162,140
    Specimen retention container
    2 View, LLC
  • U.S. Patent 8,093,030
    Thermostable viral polymerases and methods of use
    Lucigen Corporation
  • U.S. Patent 8,088,612
    Thermostable cellulase and methods of use
    C5-6 Technologies, Inc.
  • U.S. Patent 8,084,500
    Method of using catalpic acid to treat dyslipidemia
    Nutrition Therapeutics, Inc.
  • U.S. Patent 7,960,164
    Selective growth medium for Listeria spp
    Paradigm Diagnostics, Inc.
  • U.S. Patent 7,834,058
    Method of using catalpic acid to treat hyperinsulinemia
    Nutrition Therapeutics, Inc.
  • U.S. Patent 7,994,098
    Light directed DNA synthesis using inverse capping for error reduction
    Wisconsin Alumni Research Foundation
  • U.S. Patent 7,807,636 Bovine P-selectin glycoprotein ligand-1
    Wisconsin Alumni Research Foundation

Scientific Work

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Scientific Publications

  • Blasiole DA, Oler AT, Attie, AD. Regulation of apoB secretion by the LDL receptor requires exit from the cytoplasmic reticulum and interaction with apoE or apoB. J Biol Chem. 2008; 25;283(17):11374-81. (Link)
  • Ferrara CT, Wang P, Neto EC, Stevens, RD, Bain JR, Wenner BR, Ilkayeva OR, Keller MP, Blasiole DA, Kendziorski C, Yandell BS, Newgard CB, Attie AD. Genetic networks of liver metabolism revealed by integration of metabolic and transcriptional profiling. PLoS Genet. 2008; 14;4(3):e1000034. (Link)
  • Blasiole DA, Davis RA, Attie AD. The physiological and molecular regulation of lipoprotein assembly and secretion. Mol Biosyst. 2007; 3(9):608-19. (Link)
  • Nassoury N, Blasiole DA, Tebon Oler A, Benjannet S, Hamelin J, Poupon V, McPherson PS, Attie AD, Prat A, Seidah NG. The cellular trafficking of the secretory proprotein convertase PCSK9 and its dependence on the LDLR. Traffic. 2007; 8(6):718-32. ( Link)
  • Freed NE, Blasiole D, Daum L, Metzgar D, Wu J, Russell KL. Detection of influenza A, influenza B, and adenoviruses using single tube fluorogenic PCR with melting curve analysis. In Strengthening Influenza Pandemic Preparedness through Civil-Military Cooperation, edited by Neville J and Kisilev OI, pp. 123-128; 2005. Amsterdam: lOS Press.
  • Blasiole DA, Metzgar D, Daum LT, Ryan MA, Wu J, Wills C, Le CT, Freed NE, Hansen CJ, Gray GC, Russell KL. Molecular analysis of adenovirus isolates from vaccinated and unvaccinated young adults. J Clin Microbiol. 2004; 42(4): 1686-93. (Link)

Service Area Subspecialties

  • Intellectual Property
    • Biotechnology
    • Medical Devices
    • Pharmaceuticals